High-resolution ultrahigh-pressure long column reversed-phase liquid chromatography for top-down proteomics

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Reversed-phase high-performance liquid chromatography.

Micro-scale method for theophylline in body fluids by reversed-phase, high-pressure liquid chromatography.

We describe a micro-scale method for determining serum theophylline. The chromatography system includes a muBondapack C18 column and acetonitrile, 70 ml/liter of sodium acetate buffer (10 mmol/liter, ph 4.0) as the mobile phase. Test serum or plasma, 30 mul, is mixed with an equal quantity of a solution containing the internal standard, beta-hydroxyethyltheophylline in acetonitrile/sodium aceta...

Recent Advances in Ultrahigh-Pressure Liquid Chromatography

Please direct correspondence to James W. Jorgenson at [email protected]. Ultrahigh-pressure liquid chromatography (UHPLC) is a technique that is experiencing continued growth due to the benefits in separation power and speed of analysis over traditional HPLC. We attempt here to give an overview of some of the advances that have occurred in UHPLC since 2003, from the standpoint of both fund...